Thin Layer Chromatography — Contents

Table of Contents

Introduction
History
TLC Stationary Phases and Supports
Silica Gel
Reversed Phases
Alumina
Acidic Alumina
Neutral Alumina
Basic Alumina
Magnesia
Kieselguhr
Cellulose
Polyamides
Ion Exchange Materials
Coating the Thin Layer Plate
Elution Development in Thin Layer Chromatography
Choice of Phase System in TLC
Stationary Phase Selection
Choice of a Specific Ion Exchange Stationary Phase
Choice of a Specific Polar Stationary Phase.
Choice of a Specific Dispersive Stationary Phase.
Selection of the Mobile Phase
Chromatography Apparatus
Thin Layer Chromatography Chambers
Continuous Plate Development
Forced-Flow Development
Two-Dimensional TLC
Sample Application
Detection Techniques and the Quantitative Evaluation of TLC Spots
The Iodine Reagent
The Sulfuric Acid Spray
Chromic-Sulfuric Acid Spray
Fluorescence
Scanning Densitometry
Thin Layer Chromatography Applications
Measurement Methods
The Assay of Sulfamethazine in Pork Carcasses
Determination of the Platelet-Activating Factor and other Phopholipids in Human Tears
An MS Hybrid Thin Layer Plate Transport Interface for Peptide-Like Materials
Determination of Caffeine Using Both Densitometry Measurements and an Image Analyzing System
The Use of Digital Autoradiography for Biological and Drug Metabolism Studies
Purity Measurements of Phthaloyl-Amiodipine Using Over Pressurized Thin Layer Chromatography
Summary
References