Liquid Chromatography
Introduction
The Basic Liquid Chromatograph
The Mobile Phase Supply System
The Gradient Programmer
The High Pressure Programmer
The Low Pressure Programmer
The Pneumatic Pump
Non-Return Valves
The Single Piston Reciprocating Pump
The Rapid Refill Pump
The Twin-Headed Pump.
The Diaphragm Pump
The Sample Valve
Column Switching
Column Ovens
Detectors
The UV Detector
The Multi-Wavelength Detectors
The Multi-Wavelength Dispersive Detector
The Electrical Conductivity Detectors.
The Tridet Multi Functional Detector
Data Acquisition and Processing
Liquid Chromatography Stationary Phases
Silica Gel
The Preparation of Irregular Silica Gel
The Preparation of Spherical Silica Gel
The Structure of Silica Gel
The Thermogravimetric Analysis of Silica Gel
Bonded Phases
The Synthesis of Bonded Phases
Bonded Phase Synthesis by Reaction in a Solvent.
The Fluidized Bed Method for Bonded Phase Synthesis
Choosing a Bonded Phase
Types of Bonded Phase
Brush Phases
Oligomeric Phases
Bulk Phases
Interactions Between ‘Brush’ and ‘Bulk’ Reverse Phases and Aqueous Solvents
The Retention Properties of Bulk and Brush Phases.
Macroporous Polymers
LC Mobile Phases
Solvent/Solute Interactions with the Silica Gel Surface
Solute Stationary Phase Interactions
Solvent/Solute Interactions with the Reversed Phase Surface
Molecular Interactions in the Mobile Phase
Aqueous Solvent Mixtures
Chiral Stationary Phases
Macrocyclic Glycopeptide Phases
Cyclodextrin
Liquid Chromatography Applications
References