Author: RPW Scott
Book:Preparative Chromatography
Section:Preparative Packing-Preparative-Columns
Product recovery when using normal phase solvents is best carried out by bulking the fractions and removing the solvent in a rotary evaporator under reduced pressure. For reverse phase solvents that have a high water content, recovery can be best achieved by passing the fraction through a reverse phase, C18, column of high capacity. The solute and solvent is adsorbed, and the solute and solvent content of the fraction can be recovered by displacement with another solvent, and the solute, now concentrated is recovered by evaporation. Solvent Hazard Unless solvent recycling can be employed, which is not always





