Topics - Normalization
Normalization is a technique used for quantitatively assessing a chromatogram to provide a quantitative analysis of the mixture being separated. It can only be applied to processing peak areas. The quantitative results are obtained by expressing the area of a given peak as a percentage of the sum of the areas of all the peaks. This simple calculation applies to only a very limited number of analyses where the quantitative response of the detector is the same for all the eluted solutes. A typical example of this type of application is the analysis of polymers of different sizes using the refractive index detector. The refractive index of a specific polymer is constant for all polymers of that type having more than 6 monomer units.