HPLC/Fluorescence

The diastereomers of propanolol, a Beta blocker compound were separated isocratically using a GROM-SIL 100 ODS-2 FE column and detected by fluorescence at 263 and 313nm.

Amino acids in a protein hydrolysate standard (6N HCl/15 hours) were separated by gradient elution using a GROM-SIL FMOC-2 column and detected by fluorescence at 263 and 313nm.

Amino acids in an insulin hydrolysate (6N HCl/15 hours) were separated by gradient elution using a GROM-SIL FMOC-2 column and detected by fluorescence at 263 and 313nm.

Amino acids in bovine serum albumin hydrolysate (6N HCl/15 hours) were separated by gradient elution using a GROM-SIL FMOC-2 column and detected by fluorescence at 263 and 313nm.

Amino acids in human urodilatin hydrolysate (6N HCl/15 hours) were separated by gradient elution using a GROM-SIL FMOC-2 column and detected by fluorescence at 263 and 313nm.

A mixture of four aflatoxins was separated using a GROM-SIL 100 ODS-0 AB HPLC column and detected by fluorescence spectroscopy at 360nm.

A mixture of 24 amino acids was separated using a GROM-SIL OPA-2 HPLC column and detected by fluorescence spectroscopy at 230 and 445nm.

A mixture of two beta blocker drugs in human plasma was separated using a GROM-SIL 100 ODS-2 FE column and detected by fluorescence spectroscopy at 265 and 295nm.

A mixture of 16 amino acids was separated into their D and L stereoisomers using a GROM-SIL FLEC-1 column and detected by fluorescence spectroscopy at 263 and 313nm.

The amino acid proline was separated into its D and L stereoisomers using a GROM-SIL FLEC-1 column and detected by fluorescence spectroscopy at 263 and 313nm.

Fourteen amino acids in a liver biopsy sample were separated using a GROM-SIL OPA-1 column after precolumn derivatization with OPA and detected by fluorescence spectroscopy at 330 and 450nm.

Nineteen amino acids in a standard mixture were separated using a GROM-SIL OPA-1 column after precolumn derivatization with OPA /mercaptopropionic acid and detected by fluorescence spectroscopy at 330 and 450nm.

Fifteen amino acids in a protein hydrolysate were separated using a GROM-SIL OPA-1 microbore column after precolumn derivatization with OPA and detected by fluorescence spectroscopy at 330 and 450nm.

The D- and L- forms of sixteen amino acids were separated using a GROM-SIL OPA-2 column after precolumn derivatization with OPA/n-isobutryl-L-cysteine and detected by fluorescence spectroscopy at 230 and 445nm.

Eighteen amino acids in a standard were separated using a GROM-SIL FMOC 1 column after precolumn derivatization with FMOC and detected by fluorescence spectroscopy at 263 and 313nm.

Five polyamines in a decaying fish fillet were separated using a GROM-SIL Polyamine-1 column after precolumn derivatization with FMOC/EVE and detected by fluorescence spectroscopy at 263 and 310nm.

Five polyamines in fresh and spoiled meat were separated using a GROM-SIL Polyamine-1 column after precolumn derivatization with FMOC/EVE and detected by fluorescence spectroscopy at 263 and 310nm.

Seven polyamines in a meat extract were separated using a GROM-SIL Polyamine-2 column after precolumn derivatization with FMOC/EVE and detected by fluorescence spectroscopy at 263 and 310nm.

Amino acids in human urodilatin were separated after hydrolysis (6N HCl for 15 hours) using a GROM-SIL 120 ODS-5 ST column and detected by fluorescence spectroscopy at 263 and 313nm.

Two chemotherapeutic rubicins in human plasma were separated using a GROM-SIL 120 Octyl-5 CP column and detected by fluorescence spectrometry at 480 and 560nm.