Application Note Categories
HPLC/UV
In HPLC, ultraviolet (UV) detectors measure absorption of radiation from chromophores in eluted compounds over the range 190-400nm.
The detector for an HPLC is the component that emits a response due to the eluting sample compound and subsequently signals a peak on the chromatogram. It is located immediately after to the stationary phase to detect compounds as they elute from the column. Ultraviolet (UV) detectors measure the ability of chromophores in compounds in a sample to absorb UV light. This can be accomplished at one or several wavelengths over the range from 190-400nm. Fixed wavelength detectors measure at a single wavelength, usually 254nm, variable wavelength detectors measure individual wavelengths sequentially, but can detect many over a wide range and diode arrays measure a spectrum of wavelengths simultaneously. The majority of organic compounds can be analyzed by UV detectors and most HPLC analyses are performed using UV detectors.
Application Notes - HPLC/UV
The enantiomers of the pharmaceutical intermediate atrolactic acid were separated by reversed phase HPLC using a Chirobiotic Tag column and detected by UV at 230nm.
The enantiomers of the amino acid asparagine were separated by reversed phase HPLC using a Chirobiotic Tag column and detected by UV at 210nm.
The enantiomers of the amino acid alanine were separated by reversed phase HPLC using a Chirobiotic Tag column and detected by UV at 210nm.
The enantiomers of the anti-hypertensive drug alprenolol were separated by reversed phase HPLC using a Chirobiotic V column and detected by UV at 254nm.
The enantiomers of the anti-hypertensive drug atenolol were separated by reversed phase HPLC using a Chirobiotic V column and detected by UV at 230nm.
The enantiomers of the amino acid asparagine were separated by reversed phase HPLC using a Chirobiotic T column and detected by UV at 210nm.
The enantiomers of the bronchodilator drug albuterol (salbutamol) were separated by reversed phase HPLC using a Chirobiotic T column and detected by UV at 235nm.
The enantiomers of the CNS stimulant drug amphetamine were separated by reversed phase HPLC using a Cyclobond I 2000 DMP column and detected by UV at 254nm.
The enantiomers of the amino acid aspartic acid were separated by reversed phase HPLC using a Chirobiotic T column and detected by UV at 210nm.
The enantiomers of the amino acid N-CBZ-asparagine were separated by reversed phase HPLC using a Chirobiotic T column and detected by UV at 254nm.
The enantiomers of the amino acid arginine were separated by reversed phase HPLC using a Chirobiotic T column and detected by UV at 210nm.
The enantiomers of the chemical intermediate 2-bromo-3-methylbutyric acid were separated by reversed phase HPLC using a Chirobiotic R column and detected by UV at 230nm.
The enantiomers of the chemical intermediate 4-benzyl-2-oxazolidinone were separated by reversed phase HPLC using a Chirobiotic Tag column and detected by UV at 230nm.
The enantiomers of the anti-hypertensive drug bendroflumethiazide were separated by reversed phase HPLC using a Cyclobond I 2000 SN column and detected by UV at 254nm.
The enantiomers of the anti-inflammatory drug benoxaprofen were separated by reversed phase HPLC using a Chirobiotic V column and detected by UV at 254nm.
The enantiomers of the vitamin biotin were separated by reversed phase HPLC using a Chirobiotic T column and detected by UV at 210nm.
The enantiomers of the herbicide bromacil were separated by reversed phase HPLC using a Chirobiotic T column and detected by UV at 210nm.
The enantiomers of the anesthetic drug bupivacaine were separated by reversed phase HPLC using a Chirobiotic V column and detected by UV at 265nm.
The enantiomers of the analgesic drug butorphanol were separated by reversed phase HPLC using a Chirobiotic T column and detected by UV at 254nm.
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