Application Note Categories
HPLC/UV
In HPLC, ultraviolet (UV) detectors measure absorption of radiation from chromophores in eluted compounds over the range 190-400nm.
The detector for an HPLC is the component that emits a response due to the eluting sample compound and subsequently signals a peak on the chromatogram. It is located immediately after to the stationary phase to detect compounds as they elute from the column. Ultraviolet (UV) detectors measure the ability of chromophores in compounds in a sample to absorb UV light. This can be accomplished at one or several wavelengths over the range from 190-400nm. Fixed wavelength detectors measure at a single wavelength, usually 254nm, variable wavelength detectors measure individual wavelengths sequentially, but can detect many over a wide range and diode arrays measure a spectrum of wavelengths simultaneously. The majority of organic compounds can be analyzed by UV detectors and most HPLC analyses are performed using UV detectors.
Application Notes - HPLC/UV
The enantiomers of the herbicide 2-(2-chlorophenoxy) propionic acid were separated by reversed phase HPLC using a Chirobiotic Tag column and detected by UV at 230nm.
The enantiomers of the herbicide 2-(4-chlorophenoxy) propionic acid were separated by reversed phase HPLC using a Chirobiotic Tag column and detected by UV at 230nm.
The enantiomers of the amino acid carnitine were separated by reversed phase HPLC using a Chirobiotic Tag column and detected by UV at 210nm.
The enantiomers of the anti malarial drug chloroquine were separated by reversed phase HPLC using a Chirobiotic V column and detected by UV at 240nm.
The enantiomers of the anti histaminic drug chlorpheniramine were separated by reversed phase HPLC using a Cyclobond I 2000 column and detected by UV at 254nm.
The enantiomers of the diuretic drug chlorthalidone were separated by reversed phase HPLC using a Cyclobond I 2000 column and detected by UV at 254nm.
The enantiomers of the antihyperlipoproteinemic drug ciprofibrate were separated by reversed phase HPLC using a Cyclobond I 2000 column and detected by UV at 254nm.
The enantiomers of the anti depressant drug citalapam were separated by reversed phase HPLC using a Chirobiotic V column and detected by UV at 240nm.
The enantiomers of the amino acid citrulline were separated by reversed phase HPLC using a Chirobiotic Tag column and detected by UV at 210nm.
The enantiomers of the bronchodilator drug clenbuterol were separated by reversed phase HPLC using a Chirobiotic V column and detected by UV at 254nm.
The enantiomers of the rodenticide coumachlor were separated by reversed phase HPLC using a Chirobiotic V column and detected by UV at 254nm.
The enantiomers of the rodenticide coumafuryl were separated by reversed phase HPLC using a Chirobiotic V column and detected by UV at 254nm.
The enantiomers of the cresols plus the compound phenol were separated by reversed phase HPLC using a Cyclobond I 2000 column and detected by UV at 254nm.
The enantiomers of the anti neoplastic drug cyclophosphamide were separated by reversed phase HPLC using a Chirobiotic T column and detected by UV at 201nm.
The enantiomers of the amino acid cysteine were separated by reversed phase HPLC using a Chirobiotic Tag column and detected by UV at 230nm.
The enantiomers of the amino acid o-chlorophenylalanine were separated by reversed phase HPLC using a Chirobiotic T column and detected by UV at 210nm.
The enantiomers of the amino acid p-chlorophenylalanine were separated by reversed phase HPLC using a Chirobiotic T column and detected by UV at 230nm.
The enantiomers of a chiral compound used in asymmetric synthesis were separated by reversed phase HPLC using a Cyclobond I 2000 column and detected by UV at 254nm.
The enantiomers of 1,3-dicarboxylic acid allene, a chiral compound used in asymmetric synthesis were separated by reversed phase HPLC using a Chirobiotic T column and detected by UV at 254nm.
The enantiomers of the amino acid 2,3-dimethyl tyrosine were separated by reversed phase HPLC using a Chirobiotic T column and detected by UV at 210nm.
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