Application Note Categories
HPLC
High Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
HPLC or high performance liquid chromatography is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase. Both individual compounds and more complex fractions of a sample can be separated in this manner with detection possible using UV absorption, refractive index measurement, evaporative light scattering or mass spectrometry. The sample is introduced into a metal column coated with a stationary phase through which the mobile phase flows under pressure. The stronger the interaction with the stationary phase, the longer the component will be retained on the column and the stronger the interaction with the mobile phase the shorter will be its residence time on the column and vice versa. A typical separation may require 5 minutes to an hour. HPLC has found its greatest applicability in the pharmaceutical and biotechnology areas in the last 20 years due to its ability to separate complex molecules like proteins and drugs.
Application Notes - HPLC
Cholesterol separated by HPLC and detected by evaporative light scattering.
Cottonseed and linseed oils separated by HPLC into components that are detected by evaporative light scattering.
Triglycerides in olive oil were separated by reversed phase HPLC and detected by evaporative light scattering.
Six sugar standards were separated by reversed phase HPLC and detected by evaporative light scattering.
Triglycerides in a commercial lip balm, Chapstick were separated by reversed phase HPLC and detected by evaporative light scattering.
Four water-soluble vitamins were separated by reversed phase HPLC and detected by evaporative light scattering.
Five fat-soluble vitamins were separated by reversed phase HPLC and detected by evaporative light scattering.
Egg yolk phospholipids were separated by reversed phase HPLC and detected by evaporative light scattering.
Polystyrene molecular weight standards from 800 to 90,000 MW were separated by reversed phase HPLC and detected by evaporative light scattering.
Glucopyranosides in non-ionic detergent were separated by reversed phase HPLC and detected by evaporative light scattering and UV at 220nm.
The amino acid L-histidine was separated by reversed phase HPLC and detected by evaporative light scattering and UV at 220nm. The ELSD was able to resolve the L-histidine from impurities while the UV was not.
By varying the mobile phase, six fruit sugars were separated by reversed phase HPLC and detected by evaporative light scattering.
The surfactant Pluronic L-62 was separated by reversed phase HPLC and detected by evaporative light scattering.
The surfactant Plurafac B-26 was separated by reversed phase HPLC and detected by evaporative light scattering.
The surfactant DC-190 silicone based co-polymer, was separated by reversed phase HPLC and detected by evaporative light scattering.
The surfactant DC-193 silicone based co-polymer, was separated by reversed phase HPLC and detected by evaporative light scattering.
The surfactant DC-194 silicone based co-polymer, was separated by reversed phase HPLC and detected by evaporative light scattering.
The surfactant DC-1503 silicone based co-polymer, was separated by reversed phase HPLC and detected by evaporative light scattering.
Oligosaccharide mixtures used as sweeteners were separated by reversed phase HPLC and detected by evaporative light scattering.
Various sugars and sugar alcohols used as sweeteners in food products like bread, cereal and jam were separated by reversed phase HPLC and detected by evaporative light scattering.
More Application Notes from HPLC
