Application Note Categories
HPLC
High Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
HPLC or high performance liquid chromatography is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase. Both individual compounds and more complex fractions of a sample can be separated in this manner with detection possible using UV absorption, refractive index measurement, evaporative light scattering or mass spectrometry. The sample is introduced into a metal column coated with a stationary phase through which the mobile phase flows under pressure. The stronger the interaction with the stationary phase, the longer the component will be retained on the column and the stronger the interaction with the mobile phase the shorter will be its residence time on the column and vice versa. A typical separation may require 5 minutes to an hour. HPLC has found its greatest applicability in the pharmaceutical and biotechnology areas in the last 20 years due to its ability to separate complex molecules like proteins and drugs.
Application Notes - HPLC
The polysaccharides Dextran T-10 and T-40 were separated by reversed phase HPLC and detected by evaporative light scattering.
The variation in the area counts of the peaks from the separations of the surfactant n-Decyl beta-d Glucopyranoside by reversed phase HPLC and evaporative light scattering detection was determined to be less than 1%RSD.
Egg yolk phospholipids were separated by normal phase HPLC/ELSD.
Six underivatized fatty acids were separated by reversed phase HPLC/ELSD.
The non-ionic surfactant Triton X 100 was separated by HPLC/ELSD with the use of a low temperature adaptor (LTA).
Four steroids (hydrocortisone, prednisone, testosterone and progesterone) were separated by HPLC/ELSD.
Caffeine and aspirin were separated by HPLC/ELSD.
The anabolic steroids 6-B-Hydroxymethandrostenolone and methandrostenolone were separated by HPLC/ELSD.
The anabolic steroids nandrolone, 19-norepiandrosterone, 19-noretiocholanone, and 19-norandrosterone were separated by HPLC/ELSD.
The anabolic steroids testosterone and epitesterone were separated by HPLC/ELSD.
The surfactant nonphenol ethoxylate, was separated by normal phase HPLC and detected by evaporative light scattering.
The non ionic surfactant Tergitol NP-35, was separated by normal phase HPLC and detected by evaporative light scattering.
The chiral separation of d and l forms of propanolol hydrochloride was performed by reversed phase HPLC with detection by both UV and by evaporative light scattering.
The chiral separation of d and l forms of warfarin was performed by reversed phase HPLC with detection by both UV and evaporative light scattering.
Five phospholipid standards were separated by normal phase HPLC/ELSD.
Glyceryl monolaurate and its impurities were separated by reversed phase HPLC/ELSD.
Galactose was separated by reversed phase HPLC/ELSD.
Dimethicone standard was separated by reversed phase HPLC/ELSD, but separation from a hand lotion was only partly successful due to interferences.
Three pharmaceutical stimulants, theophylline, caffeine and ethamivan were separated by reversed phase HPLC/ELSD.
A mixture of four steroids, progesterone, testosterone, 17-hydroxyprogesterone and hydrocortisone was separated by reversed phase HPLC/ELSD using supercritical fluid chromatography.
More Application Notes from HPLC
