Application Notes
Cyclophosphamide by HPLC/UV
The enantiomers of the anti neoplastic drug cyclophosphamide were separated by reversed phase HPLC using a Chirobiotic T column and detected by UV at 201nm.
Cyclophosphamide, Astec Application Report NEED
Cyclophosphamide, CAS No. 50-18-0, an anti neoplastic drug was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using ethanol, THF, water (5:15:90). The enantiomers were detected by UV at 201nm.
Technique
HPLC/UVIn HPLC, ultraviolet (UV) detectors measure absorption of radiation from chromophores in eluted compounds over the range 190-400nm.
Source
Astec (Advanced Separation Technologies, Inc.)Astec (Advanced Separation Technologies) is a leader in the production and sale of chiral chromatography columns for LC and capillary GC.
