Chlorpheniramine by HPLC/UV

The enantiomers of the anti histaminic drug chlorpheniramine were separated by reversed phase HPLC using a Cyclobond I 2000 column and detected by UV at 254nm.

Chlorpheniramine, Astec Application Report 4003

Chlorpheniramine, CAS No. 132-22-9, an anti histaminic drug was separated into its two respective enantiomers by isocratic elution in around 20 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 column (250 x 4.6mm, Part No. 20024) using water, acetonitrile, (10:90), 1% TEAA, pH 4.1. The enantiomers were detected by UV at 254nm.