Application Notes
Chloroquine by HPLC/UV
The enantiomers of the anti malarial drug chloroquine were separated by reversed phase HPLC using a Chirobiotic V column and detected by UV at 240nm.
Chloroquine, Astec Application Report 111
Chloroquine, CAS No. 54-05-7, an anti malarial drug was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using water, methanol, acetic acid, TEA (100:1:1). The enantiomers were detected by UV at 240nm.
Technique
HPLC/UVIn HPLC, ultraviolet (UV) detectors measure absorption of radiation from chromophores in eluted compounds over the range 190-400nm.
Source
Astec (Advanced Separation Technologies, Inc.)Astec (Advanced Separation Technologies) is a leader in the production and sale of chiral chromatography columns for LC and capillary GC.
