Bupivacaine by HPLC/UV

The enantiomers of the anesthetic drug bupivacaine were separated by reversed phase HPLC using a Chirobiotic V column and detected by UV at 265nm.

Bupivacaine, Astec Application Report 110

Bupivacaine, CAS No. 2180-92-9, an anesthetic drug was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using water, methanol (95:5), 1% TEAA, pH 4.1. The enantiomers were detected by UV at 265nm.