Application Notes
Biotin by HPLC/UV Using Chirobiotic T Column
The enantiomers of the vitamin biotin were separated by reversed phase HPLC using a Chirobiotic T column and detected by UV at 210nm.
Biotin, Astec Application Report 240
Bitoin, CAS No. 58-85-5, a vitamin was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using water, methanol (40:60), 0.1% sodium phosphate, pH 4.1. The enantiomers were detected by UV at 210nm.
Technique
HPLC/UVIn HPLC, ultraviolet (UV) detectors measure absorption of radiation from chromophores in eluted compounds over the range 190-400nm.
Source
Astec (Advanced Separation Technologies, Inc.)Astec (Advanced Separation Technologies) is a leader in the production and sale of chiral chromatography columns for LC and capillary GC.
