Application Notes
Baseline Resolution of EPA Method 608 Pesticides
Sixteen EPA Method 608 pesticides were baseline resolved in 17 minutes on a capillary column by GC/ECD.
Achieving Baseline Resolution of EPA Method 608 Pesticides with Alltech Pesticide Capillary, Alltech Application Note ANE005, 1997.
To demonstrate baseline resolution of all EPA Method 608 pesticides for the EPA Contract Laboratory Program (CLP), a capillary column, Alltech Pesticide Capillary (part No. 13662) 30m x 0.25mm x 0.25µm was installed on a GC equipped with an electron capture detector (ECD).
A mixture of pesticides were injected: a-BHC, g-BHC (lindane), b-BHC, d-BHC, heptachlor, aldrin, heptachlor epoxide, endosulfan I, p,p’-DDE, dieldrin, endrin, p,p’-DDD, Endosulfan II, p,p’-DDT (DDT), endrin aldehyde and endosulfan sulfate.
The column temperature was increased from 190°C to 240°C at 6°C/min. All 16 pesticides eluted within 17 minutes with baseline separation, including four typically difficult to resolve compounds baseline separated: beta-BHC resolved from gamma-BHC and p,p’-DDD resolved from Endosulfan II.
Key Analyte(s)
a-BHC
aldrin
b-BHC
d-BHC
dieldrin
endosulfan I
endosulfan II
endosulfan sulfate
endrin
More AnalytesEquipment
Alltech Heliflex® AT-Pesticide Capillary ColumnsAlltech Heliflex® AT-Amino Acid capillary column are a line of intermediate polarity fused silica GC columns manufactured and sold by Alltech Associates designed to provide unique selectivity for pesticide confirmation.
Technique
GC/ECDThe ECD or electron capture detector measures electron capturing compounds (usually halogenated) by creating an electrical field in which molecules exiting a GC column can be detected by the drop in current in the field.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
