Bendroflumethiazide by HPLC/UV-Cyclobond I 2000 SN Column

The enantiomers of the anti-hypertensive drug bendroflumethiazide were separated by reversed phase HPLC using a Cyclobond I 2000 SN column and detected by UV at 254nm.

Bendroflumethiazide, Astec Application Report 4002

Bendroflumethiazide, CAS No. 73-48-3, an anti-hypertensive drug was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 SN column (250 x 4.6mm, Part No. 20524) using water, hexane (30:70) 1% TEAA, pH 4.5. The enantiomers were detected by UV at 254nm.