Application Notes
2-bromo-3-methylbutyric acid by HPLC/UV
The enantiomers of the chemical intermediate 2-bromo-3-methylbutyric acid were separated by reversed phase HPLC using a Chirobiotic R column and detected by UV at 230nm.
2-bromo-3-methylbutyric acid, Astec Application Report 3000
2-bromo-3-methylbutyric acid, CAS No. 565-74-2, a chemical intermediate was separated into its two respective enantiomers by isocratic elution in around 20 minutes by reversed phase HPLC on an Astec Chirobiotic R column (250 x 4.6mm, Part No. 13024) using ethanol, acetic acid, hexane and TEA (60:40:0.02:0.05). The enantiomers were detected by UV at 230nm.
Technique
HPLC/UVIn HPLC, ultraviolet (UV) detectors measure absorption of radiation from chromophores in eluted compounds over the range 190-400nm.
Source
Astec (Advanced Separation Technologies, Inc.)Astec (Advanced Separation Technologies) is a leader in the production and sale of chiral chromatography columns for LC and capillary GC.
