Application Notes
The enantiomers of the amino acid N-CBZ-asparagine were separated by reversed phase HPLC using a Chirobiotic T column and detected by UV at 254nm.
N-CBZ-Asparagine, Astec Application Report 203
N-CBZ-Asparagine, CAS No. NR, an amino acid was separated into its two respective enantiomers by isocratic elution (time not given) by reversed phase HPLC on an Astec Chirobiotic T column (250 x 4.6mm, Part No. 12024) using methanol, water, at pH 4.1 with 1% TEAA (20:80). The enantiomers were detected by UV at 254nm.
In HPLC, ultraviolet (UV) detectors measure absorption of radiation from chromophores in eluted compounds over the range 190-400nm.
Astec (Advanced Separation Technologies) is a leader in the production and sale of chiral chromatography columns for LC and capillary GC.