Application Notes
Amphetamine by HPLC/UV
The enantiomers of the CNS stimulant drug amphetamine were separated by reversed phase HPLC using a Cyclobond I 2000 DMP column and detected by UV at 254nm.
Amphetamine, Astec Application Report 4000
Amphetamine, CAS No. 300-62-9, a CNS stimulant drug was separated into its two respective enantiomers after derivatization with AQC by isocratic elution in around 20 minutes by reversed phase HPLC on an Astec Cyclobond I 2000 DMP column (250 x 4.6mm, Part No. 20724) using water, acetonitrile, TEAA at pH 4. (45:55:0.01). The enantiomers were detected by UV at 230nm.
Technique
HPLC/UVIn HPLC, ultraviolet (UV) detectors measure absorption of radiation from chromophores in eluted compounds over the range 190-400nm.
Source
Astec (Advanced Separation Technologies, Inc.)Astec (Advanced Separation Technologies) is a leader in the production and sale of chiral chromatography columns for LC and capillary GC.
