Application Notes
Alprenolol by HPLC/UV Using Chirobiotic V Column
The enantiomers of the anti-hypertensive drug alprenolol were separated by reversed phase HPLC using a Chirobiotic V column and detected by UV at 254nm.
Alprenolol, Astec Application Report 101
Alprenolol, CAS No. 13655-52-2, an anti-hypertensive drug was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic V column (250 x 4.6mm, Part No. 11024) using methanol, acetic acid, TEA (100:0.01:0.01). The enantiomers were detected by UV at 254nm.
Technique
HPLC/UVIn HPLC, ultraviolet (UV) detectors measure absorption of radiation from chromophores in eluted compounds over the range 190-400nm.
Source
Astec (Advanced Separation Technologies, Inc.)Astec (Advanced Separation Technologies) is a leader in the production and sale of chiral chromatography columns for LC and capillary GC.
