Application Notes
Alanine by HPLC/UV Using Chirobiotic Tag Column
The enantiomers of the amino acid alanine were separated by reversed phase HPLC using a Chirobiotic Tag column and detected by UV at 210nm.
Alanine, Astec Application Report 5001
Alanine, CAS No. 56-41-7, an amino acid was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol, water (30:70). The enantiomers were detected by UV at 210nm.
Technique
HPLC/UVIn HPLC, ultraviolet (UV) detectors measure absorption of radiation from chromophores in eluted compounds over the range 190-400nm.
Source
Astec (Advanced Separation Technologies, Inc.)Astec (Advanced Separation Technologies) is a leader in the production and sale of chiral chromatography columns for LC and capillary GC.
