Application Notes
Atrolactic Acid by HPLC/UV Using Chirobiotic Tag Column
The enantiomers of the pharmaceutical intermediate atrolactic acid were separated by reversed phase HPLC using a Chirobiotic Tag column and detected by UV at 230nm.
Atrolactic Acid, Astec Application Report 5003
Atrolactic acid, CAS No. 515-30-0, an organic intermediate used in pharmaceutical manufacturing was separated into its two respective enantiomers by isocratic elution in around 10 minutes by reversed phase HPLC on an Astec Chirobiotic Tag column (250 x 4.6mm, Part No. 14024) using methanol, acetic acid, TEA (100:0.1:0.1). The enantiomers were detected by UV at 230nm.
Technique
HPLC/UVIn HPLC, ultraviolet (UV) detectors measure absorption of radiation from chromophores in eluted compounds over the range 190-400nm.
Source
Astec (Advanced Separation Technologies, Inc.)Astec (Advanced Separation Technologies) is a leader in the production and sale of chiral chromatography columns for LC and capillary GC.
