Application Notes
Carbohydrates in Infant Formula by HPLC/ELSD
Carbohydrate sugars in soy infant formula were determined by reversed phase HPLC with evaporative light scattering detection.
Analysis of Carbohydrates in Soy Infant Formula, Alltech Application Note 0044E, March 29, 2004.
Carbohydrate sugars in soy infant formula were determined by reversed phase HPLC with evaporative light scattering detection. The fats in the infant formula were first extracted using hexane and other cleanup procedures performed prior to the HPLC analysis.
Glucose, sucrose, maltose and raffinose were separated isocratically and by gradient elution with acetonitrile/water on an Alltima Amino, 5µm, 250 x 4.6mm column (Part No. 88217) and detected by evaporative light scattering using an Alltech MKIII ELSD (Evaporative Light Scattering Detector.) The isocratic elution took 16 minutes while the gradient elution took around 12.
The ELSD was found to be more sensitive than refractive index detection and its response linear for these sugars.
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
glucose (dextrose)Glucose (dextrose)is a simple sugar used in foods and drinks. It is most often measured using HPLC and GC/MS.
maltose
raffinose
sucrose
Equipment
Alltech Alltima Reversed-Phase ColumnsAlltech Alltima Reversed Phase columns are a line of HPLC columns in the Alltech HP family. The Alltech Alltima family are high quality general purpose columns. They are made with spherical silica, polymerically bonded and double endcapped for long life
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
