Application Notes
Acetone as Solvent for Carbohydrates by HPLC/ELSD
The use of acetone in the mobile phase to speed up run times for carbohydrates in beer by reversed phase HPLC and evaporative light scattering detection is described.
Alternative Solvents for Carbohydrate Analyses by HPLC, Alltech Application Note 0054E, March 29, 2004.
The use of acetone in the mobile phase to speed up run times for carbohydrates in beer by reversed phase HPLC and evaporative light scattering detection is described.
Seven sugars and oligomers in a beer standard (fructose, glucose, sucrose, maltose, maltotriose, maltotetraose and maltopentose) were separated by gradient elution with acetonitrile/water and with acetonitrile/acetone and water on a Prevail Carbohydrate ES, 5µm, 250 x 4.6mm column (Part No. 35101) and detected by evaporative light scattering using an Alltech 2000 ELSD (Evaporative Light Scattering Detector.) The separation using the unmodified mobile phase took 35 minutes, while that using the acetone took only 18.
Likewise, a mixture of fructose, mannitol, glucose, sucrose, raffinose and stachyose was separated in 16 minutes using the acetone modifier and a similar elution gradient.
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
fructose
glucose (dextrose)Glucose (dextrose)is a simple sugar used in foods and drinks. It is most often measured using HPLC and GC/MS.
maltopentaose
maltose
maltotetraose
maltotriose
mannitol
raffinose
stachyose
Equipment
Alltech Prevail Carbohydrate ES ColumnsAlltech Prevail Carbohydrate ES columns are a line of polymer based reversed phase HPLC columns in the Alltech Prevail family for use in determining carbohydrates including mono- and oligosaccharides and sugar alcohols. They are available with a single p
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
