Application Notes
Detecting Adulteration in Orange Juice by HPLC/ELSD
Adulteration of orange juice with high fructose corn syrup detected by reversed phase HPLC with evaporative light scattering.
Orange Juice Profiles, Alltech Application Note 0052E, January 15, 2002.
The ratio of glucose, fructose and sucrose in pure orange juice is well known. Adulteration with high fructose corn syrup is sometimes done. To detect this form of adulteration, an HPLC application with evaporative light scattering detection was developed and described. These sugars in Minute Maid 100% OJ, Dominick’s 100% OJ and Dominick’s Orange Drink were separated isocratically with acetonitrile/water on a Prevail Carbohydrate ES, 53 x 7mm Rocket column (Part No. 35104) and detected by evaporative light scattering using an Alltech 2000 ELSD (Evaporative Light Scattering Detector.)
Fructose, glucose and sucrose in the pure orange juices were all found at about the same ratio, while the levels of fructose and glucose were nearly 3 times higher in the Orange Drink and no sucrose was found. Although the orange drink in this study was not an adulterated product, the application shows how such adulteration could be detected.
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
fructose
glucose (dextrose)Glucose (dextrose)is a simple sugar used in foods and drinks. It is most often measured using HPLC and GC/MS.
sucrose
Equipment
Alltech Prevail Carbohydrate ES ColumnsAlltech Prevail Carbohydrate ES columns are a line of polymer based reversed phase HPLC columns in the Alltech Prevail family for use in determining carbohydrates including mono- and oligosaccharides and sugar alcohols. They are available with a single p
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
