Application Notes
Sugars in Fruit Juices by HPLC/ELSD
Simple sugars and sugar alcohols found in fruit juices were separated by reversed phase HPLC and detected by evaporative light scattering.
Carbohydrate Profiles-Fruit Juice, Alltech Application Note 0053E, March 29, 2004.
Simple sugars and sugar alcohols found in fruit juices were separated isocratically and by gradient elution by reversed phase HPLC on several different HPLC columns with acetonitrile/water and detected by evaporative light scattering using an Alltech 2000 ELSD (Evaporative Light Scattering Detector.)
Fructose, glucose and sucrose in orange juice were separated isocratically in around 15 minutes on a Prevail Carbohydrate ES, 5µm, 250 x 4.6mm column (Part No. 35101).
Fructose, mannitol, glucose and sucrose in nectarine juice were separated by gradient elution in 7 minutes on a Prevail Carbohydrate ES, 100 x 7mm Rocket column (Part No. 35103.)
Fructose, mannitol, glucose and sucrose and raffinose in plum juice were separated by gradient elution in 7 minutes on a Prevail Carbohydrate ES, 100 x 7mm Rocket column (Part No. 35103.)
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
fructose
glucose (dextrose)Glucose (dextrose)is a simple sugar used in foods and drinks. It is most often measured using HPLC and GC/MS.
mannitol
raffinose
sucrose
Equipment
Alltech Prevail Carbohydrate ES ColumnsAlltech Prevail Carbohydrate ES columns are a line of polymer based reversed phase HPLC columns in the Alltech Prevail family for use in determining carbohydrates including mono- and oligosaccharides and sugar alcohols. They are available with a single p
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
