Application Notes
Beer Oligomers by HPLC/ELSD
Oligosaccharides found in beer were separated by reversed phase HPLC and detected by evaporative light scattering.
Carbohydrate Profiles-Beer, Alltech Application Note 0055E, January 18, 2002.
Oligosaccharides found in beer were separated by gradient elution in around 45 minutes by reversed phase HPLC on a Prevail Carbohydrate ES, 5µm, 250 x 4.6mm column (Part No. 35101) using acetonitrile/water and detected by evaporative light scattering using an Alltech 2000 ELSD (Evaporative Light Scattering Detector).
Frucotose, glucose, maltose, maltotriose, maltopentaose and maltohexaose were separated in New Castle Ale and maltotriose, maltohexaose and maltoheptaose were separated in Goose Island Beer.
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
fructose
glucose (dextrose)Glucose (dextrose)is a simple sugar used in foods and drinks. It is most often measured using HPLC and GC/MS.
maltoheptaose
maltohexaose
maltopentaose
maltose
maltotriose
Equipment
Alltech Prevail Carbohydrate ES ColumnsAlltech Prevail Carbohydrate ES columns are a line of polymer based reversed phase HPLC columns in the Alltech Prevail family for use in determining carbohydrates including mono- and oligosaccharides and sugar alcohols. They are available with a single p
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
