Application Notes
Fatty Acid Methyl Esters by HPLC/ELSD
The ELSD and UV detection of five fatty acid methyl esters separated by reversed phase HPLC was compared.
Fatty Acid Methyl Esters, Alltech Application Note 0038E, July 10, 2000.
The ELSD and UV detection of five fatty acid methyl esters (FAME) separated by reversed phase HPLC was compared. The FAME compounds were separated isocratically in around 10 minutes by reversed phase HPLC on a Silver Impregnated Ion-Exchange, 250 x 4.6mm column (Part No. not given) using acetonitrile/methanol and detected by evaporative light scattering (ELSD) using an Alltech Model 500 (Evaporative Light Scattering Detector) and by UV at 206 nm.
All five of the FAME compounds (18:0, 18:1 n-9, 18:2, n-6, 18:3 n-3 and 20:4 n-6) were resolved by ELSD, but only the last 3 were resolved by UV.
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
18:0
18:1 n-9
18:2 n-6
18:3 n-3
20:4 n-6
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
