Application Notes
Edible Oil Adulteration by HPLC/ELSD
To detect adulteration of olive oil with vegetable oil by measuring triglycerides, a reversed phase HPLC procedure with ELSD detection was developed.
Edible Oil Adulteration, Alltech Application Note 0056E, March 29, 2004.
To detect adulteration of olive oil with vegetable oil by measuring triglycerides, a reversed phase HPLC procedure with ELSD detection was developed. Triglycerides in pure extra virgin olive oil, vegetable oil, a common adulterant of olive oils and a 50:50 mixture of the two oils were separated by gradient elution in around 15 minutes by reversed phase HPLC on an Alltima C18, 3µm, 250 x 4.6mm column (Part No. 81387) using methylene choride/acetonitrile and were detected by evaporative light scattering using an Alltech Model 2000 (Evaporative Light Scattering Detector.)
The fatty acids capric acid, lauric acid, myristic acid, palmitic acid, palmitoleic acid, stearic acid, oleic acid (O), linoleic acid (L) and linolenic acid when combined together produce various triglycerides. The HPLC separations showed that the vegetable oil adulteration could be easily detected by the presence of the triglyceride trilinolein (LLL) that is significant in vegetable oil and the reduced presence of triolein (OOO) which is significant in olive oil.
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
capric acid
lauric acid
linoleic acid
linolenic acid
LLL
LLO
myristic acid
oleic acid
palmitic acidPalmitic acid is a naturally occurring fatty acid also produced commercially from palm and coconut oil. It is used is soaps, cosmetics and food additives. It is most often measured using HPLC, GC/FID and GC/MS.
Equipment
Alltech Alltima Normal Phase ColumnsAlltech Alltima Normal Phase columns are a line of HPLC columns in the Alltech HP family. The Alltech Alltima family are high quality general purpose columns. They are made with spherical silica, polymerically bonded and double endcapped for long lifeti
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
