Application Notes
Polyethylene Glycol 600 by HPLC/ELSD
Polyethylene glycol MW 600 was separated by reversed phase HPLC and detected by ELSD.
Polymer-Polyethylene Glycol, Alltech Application Note 0029E, July 7, 2000.
Polyethylene glycol MW 600 was separated by gradient elution in around 30 minutes by reversed phase HPLC on an Adsorbosphere C8, 5µm, 250 x 4.6mm column (Part No. 287112) using water/methanol and detected by evaporative light scattering (ELSD) using an Alltech Model 500 (Evaporative Light Scattering Detector.). To achieve a good separation, the column and the tubing leading to it were heated with a preheating coil set at 70ºC.
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
Polyethylene glycol MW 600
Equipment
Alltech Adsorbosphere ColumnsAlltech Adsorbosphere® Columns are designed using high temperature bonding for exceptional surface coverage and stability for common applications. They are available in pore sizes from 60-90Å and come in four varieties.
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
