Application Notes
Ginseng Root Compounds by HPLC/ELSD
Ginseng root extracts were separated by reversed phase HPLC and detected by ELSD.
Analysis of Ginsenosides by HPLC with ELSD, Alltech Application Note 0051E, July 7, 2000.
Ginseng root extracts, known as gensenosides and crude ginseng root powder were separated by gradient elution in around 20 minutes by reversed phase HPLC on a Partisil ODS-3, 5µm, 250 x 4.6mm column (Part No. 8660) using water/acetonitrile. The ginsenosides Rg1 and Rb1 from a standard mixture and peaks corresponding to them in the crude root powder were detected by evaporative light scattering using an Alltech Model 500 (Evaporative Light Scattering Detector.)
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
ginsenoside Rg1
ginsenosides Rb1
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
