Application Notes
Muscle Relaxant Drugs by HPLC/ELSD
A mixture of four muscle relaxant drugs, rocuronium, pancuromium, pipecuronium and vecuronium bromide was separated by reversed phase HPLC and the drugs detected by ELSD, UV and refractive index.
A mixure of four muscle relaxant drugs, rocuronium, pancuromium, pipecuronium and vecuronium bromide was separated by gradient elution in around 15 minutes by reversed phase HPLC on an Adsorbosphere UHS C18, 5µm, 150 x 2.1mm column (Part No. 288435) using water/methanol. The drugs were detected by evaporative light scattering using an Alltech Model 500 (Evaporative Light Scattering Detector), by UV at 205 nm and by refractive index (RI). The ELSD baseline was more stable than that from the UV and RI determinations. The HPLC/ELSD detection limit was 9ng on column.
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
pancuromium bromide
pipecuronium bromide
rocuronium bromide
vecuronium bromide
Equipment
Alltech Adsorbosphere ColumnsAlltech Adsorbosphere® Columns are designed using high temperature bonding for exceptional surface coverage and stability for common applications. They are available in pore sizes from 60-90Å and come in four varieties.
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
