Application Notes
Galactose Standard by Reversed Phase HPLC/ELSD
Galactose was separated by reversed phase HPLC/ELSD.
Galactose, Alltech Application Note 0043E, May 10, 2000.
Galactose was separated isocratically in around 10 minutes by reversed phase HPLC on an Alltech 700 CH Carbohydrate, 300 x 6.5mm column (Part No. 70057) using water and detected by evaporative light scattering using an Alltech 500 ELSD (Evaporative Light Scattering Detector). A linear response was found with a detection limit of 10µg/mL.
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
galactose
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
