Application Notes
Glyceryl Monolaurate Purity by Reversed Phase HPLC/ELSD
Glyceryl monolaurate and its impurities were separated by reversed phase HPLC/ELSD.
Monoglyceride Purity - Glyceryl Monolaurate, Alltech Application Note 0037E, April 21, 2000.
Glyceryl monolaurate, a monoglyceride and its impurities were separated isocratically in around 10 minutes by reversed phase HPLC on a Nucleosil C18, 5µm, 250 x 4.6mm column (Part No. 89141) using acetonitrile and detected by evaporative light scattering using an Alltech 500 ELSD (Evaporative Light Scattering Detector).
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
glyceryl monolaurate
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
