Application Notes
Purity of Phospholipid Standards by Normal Phase HPLC/ELSD
Five phospholipid standards were separated by normal phase HPLC/ELSD.
Phospholipid Class Standards- Normal Phase, Alltech Application Note 0036E, July 7, 2000.
Five phospholipid standards were separated by normal phase HPLC/ELSD.
Separation of a mixture of phospholipid standards was achieved by gradient elution with IPA/hexane/water/ammonium hydroxide in around 14 minutes using an Alltech Allsphere Silica Hypersil, 3µm, 100 x 4.6mm column (Part No. 778382) with detection by evaporative light scattering using an Alltech 500 ELSD (Evaporative Light Scattering Detector). Phosphatidylinositol, phosphatidic acid, phosphatidylserine, phosphatidylcholine and sphingomyelin were separated.
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
phosphatidic acid
phosphatidylcholine
phosphatidylinositol
phosphatidylserine
phospholipids
sphingomyelin
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
