Application Notes
Chiral Separation of Warfarin Enantiomers by HPLC/ELSD
The chiral separation of d and l forms of warfarin was performed by reversed phase HPLC with detection by both UV and evaporative light scattering.
Chiral Separation of Warfarin Enantiomers, Alltech Application Note 0032E, May 2, 2000.
The chiral separation of d and l forms of warfarin was performed by reversed phase HPLC using isocratic elution in around 6 minutes on an Astec Cyclobond 1 2000, 5µm, 250 x 4.6mm column (Part No. 410101) using acetic acid/TEA and acetonitrile/methanol. Detection was achieved both UV at 254 nm and by evaporative light scattering using an Alltech 500 ELSD (Evaporative Light Scattering Detector).
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
d-warfarin
l-warfarin
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
