Application Notes
Nonphenol Ethoxylate Surfactant by HPLC/ELSD
The surfactant nonphenol ethoxylate, was separated by normal phase HPLC and detected by evaporative light scattering.
Surfactant Nonphenol Ethoxylate, Alltech Application Note 0039E, July 10, 2000.
The surfactant nonphenol ethoxylate was separated by gradient elution in around 25 minutes by normal phase HPLC on a Supelco LC-DIOL, 250 x 4.6mm column (Part No. 88133) using hexane/methylene chloride and hexane/methanol and detected by evaporative light scattering using an Alltech 500 ELSD (Evaporative Light Scattering Detector).
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
non phenol ethoxylate
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
