Application Notes
6-B-Hydroxymethandrostenolone & methandrostenolone by HPLC/ELSD
The anabolic steroids 6-B-Hydroxymethandrostenolone and methandrostenolone were separated by HPLC/ELSD.
Anabolic Steroids- 6-B-Hydroxymethandrostenolone and methandrostenolone, Alltech Application Note 0034E, May 14, 2003.
The anabolic steroids B-Hydroxymethandrostenolone and methandrostenolone were separated by HPLC/ELSD.
Separation of the anabolic steroids B-Hydroxymethandrostenolone and methandrostenolone was achieved by reversed phase isocratic elution with acetonitile/water in around 6 minutes using an Adsorbosphere C8, 5µm, 250 x 4.6mm column (Part No. 287112). Detection was by evaporative light scattering using an Alltech 500 ELSD (Evaporative Light Scattering Detector).
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
6-B-Hydroxymethandrostenolone
methandrostenolone
Equipment
Alltech Adsorbosphere ColumnsAlltech Adsorbosphere® Columns are designed using high temperature bonding for exceptional surface coverage and stability for common applications. They are available in pore sizes from 60-90Å and come in four varieties.
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
