Application Notes
Caffeine and Aspirin by HPLC/ELSD
Caffeine and aspirin were separated by HPLC/ELSD.
Pharmaceutical-Caffeine and Aspirin, Alltech Application Note 0028LE, June 23, 2000.
Caffeine and aspirin were separated by HPLC/ELSD.
Separation of caffeine and aspirin was achieved by reversed phase isocratic elution with water/methanol/acetic acid in around 2 minutes using an Econosphere C18, 3µm, 30 x 4.6mm column (Part No. 28213). Detection was by evaporative light scattering using an Alltech 500 ELSD (Evaporative Light Scattering Detector).
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
aspirin (acetylsalicylic acid)Aspirin (acetylsalicylic acid, the most widely used over the counter drug, is an anti-inflammatory agent and analgesic. It is most often measured using HPLC.
caffeineCaffeine, a coffee extract acts as a stimulant on the central nervous system and long term use can lead to dependency. It is most often meaasured using HPLC, GC/FID and GC/MS.
Equipment
Alltech Econosphere ColumnsAlltech Econosphere Columns are low-cost columns for common applications. They are available in 80Å pore size and are acid-washed for low metal content. They are available with eleven packing phases.
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
