Application Notes
Underivatized C14-C18 Fatty Acids by HPLC/ELSD
Six underivatized fatty acids were separated by reversed phase HPLC/ELSD.
Fatty Acids (Underivatized), Alltech Application Note 0025E, June 5, 2000.
Separation of a mixture of six underivatized fatty acid standards was achieved by gradient elution with water/acetonitrile in around 20 minutes using an Alltech Alltima C18-LL, 5µm, 250 x 2.1mm column (Part No. 88389). Detection was by evaporative light scattering using an Alltech 500 ELSD (Evaporative Light Scattering Detector).
Linolenic acid, myristic acid, linoleic acid, palmitic acid, oleic acid and stearic acid were separated.
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
linoleic acid
linolenic acid
myristic acid
oleic acid
palmitic acidPalmitic acid is a naturally occurring fatty acid also produced commercially from palm and coconut oil. It is used is soaps, cosmetics and food additives. It is most often measured using HPLC, GC/FID and GC/MS.
stearic acidStearic acid, prepared by hydrogenation of cottonseed oil, is used in manufacturing soaps and greases. It is most often measured using HPLC, GC/FID and GC/MS.
Equipment
Alltech Alltima Reversed-Phase ColumnsAlltech Alltima Reversed Phase columns are a line of HPLC columns in the Alltech HP family. The Alltech Alltima family are high quality general purpose columns. They are made with spherical silica, polymerically bonded and double endcapped for long life
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
