Application Notes
Egg Yolk Phospholipids by Normal Phase HPLC/ELSD
Egg yolk phospholipids were separated by normal phase HPLC/ELSD.
Phospholipids- Normal Phase, Alltech Application Note 0024E, May 10, 2000.
Egg yolk phospholipids and phospholipid standards were separated by normal phase HPLC/ELSD.
Separation of a mixture of phospholipid standards was achieved by gradient elution with IPA/hexane/water in around 14 minutes using an Alltech Allsphere Silica Hypersil, 3µm, 100 x 4.6mm column (Part No. 778382) and detection was by evaporative light scattering using an Alltech 500 ELSD (Evaporative Light Scattering Detector). Cholesterol, palmitic acid, phosphatidylethanolamine, phosphatidylserine, phosphatidylcholine and sphingomyelin were separated.
Separation of egg yolk phospholipids was achieved by gradient elution with IPA/hexane/water in around 14 minutes using a Spherisorb Silica, 3µm, 100 x 4.6mm column (Part No. 8382) and detection was by evaporative light scattering using an Alltech 500 ELSD (Evaporative Light Scattering Detector). Cholesterol, phosphatidylethanolamine, phosphatidylcholine and sphingomyelin were separated.
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
cholesterolCholesrerol is a steroid produced naturally and obtained from animals in the diet. Its level in the blood is associated with cardiovascular disease. It is most often measured using HPLC, GC/FID and GC/MS.
palmitic acidPalmitic acid is a naturally occurring fatty acid also produced commercially from palm and coconut oil. It is used is soaps, cosmetics and food additives. It is most often measured using HPLC, GC/FID and GC/MS.
phosphatidylcholine
phosphatidylethanolamine
phosphatidylserine
sphingomyelin
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
