Application Notes
Precision of n-Decyl beta-d Glucopyranoside by HPLC/ELSD
The variation in the area counts of the peaks from the separations of the surfactant n-Decyl beta-d Glucopyranoside by reversed phase HPLC and evaporative light scattering detection was determined to be less than 1%RSD.
Reproducibility- n-Decyl beta-d Glucopyranoside, Alltech Application Note 0022E, May 2, 2000.
The variation in the area counts of the peaks from 11 separations of the surfactant n-Decyl beta-d Glucopyranoside by reversed phase HPLC and evaporative light scattering detection was determined to be less than 1%RSD.
Separation was achieved isocratically with methanol/water by reversed phase HPLC in around 3 minutes using an Alltech Econosphere C18, 5µm, 150 x 4.6mm column (Part No. 70065) and detection was by evaporative light scattering using an Alltech 500 ELSD (Evaporative Light Scattering Detector).
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
n-decyl-beta-d-glucopyranoside
Equipment
Alltech Econosphere ColumnsAlltech Econosphere Columns are low-cost columns for common applications. They are available in 80Å pore size and are acid-washed for low metal content. They are available with eleven packing phases.
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
