Application Notes
Polysaccharides Dextran T-10 and T-40 by HPLC/ELSD
The polysaccharides Dextran T-10 and T-40 were separated by reversed phase HPLC and detected by evaporative light scattering.
Polysaccharides, Alltech Application Note 0021E, May 2, 2000.
The polysaccharides Dextran T-10 and T-40 were separated isocratically by reversed phase HPLC in around 10 minutes on an Alltech Macrosphere GPC 100, 7µm, 250 x 4.6mm column (Part No. 88133) using water at pH 8.0 and detected by evaporative light scattering using an Alltech 500 ELSD (Evaporative Light Scattering Detector).
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
Dextran T-10
Dextran T-40
Equipment
Alltech Macrosphere ColumnsThe Alltech Macrosphere Columns are designed to separate proteins, peptides and polar synthetic compounds by size-exclusion. They are silica based (60-300Å).
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
