Application Notes
Sugars in Food Products by HPLC/ELSD
Various sugars and sugar alcohols used as sweeteners in food products like bread, cereal and jam were separated by reversed phase HPLC and detected by evaporative light scattering.
Carbohydrate Profiles-Representative Food Products, Alltech Application Note 0020E, March 29, 2004.
Sugars in corn syrup and sugar alcohols used as sweeteners in food products were separated isocratically and by gradient elution by reversed phase HPLC on several different HPLC columns with acetonitrile/water and detected by evaporative light scattering using an Alltech 2000 ELSD (Evaporative Light Scattering Detector.)
A mixture of sugars and sugar alcohols was separated by gradient elution in around 55 minutes on a Prevail Carbohydrate ES, 5µm, 250 x 4.6mm column (Part No. 35101).
Iso-erythritol, arabitol, fructose, sorbitol, mannitol, glucose, sucrose, maltitol, maltose, raffinose, maltotriose and stachyose were separated.
Iso-erythritol, xylitol, mannitol and maltitol were separated isocratically in around 10 minutes on a Prevail Carbohydrate ES, 5µm, 250 x 4.6mm column (Part No. 35101).
Mannitol, glucose and sucrose in soy breakfast cereal were separated by gradient elution in 7 minutes on a Prevail Carbohydrate ES, 100 x 7mm Rocket column (Part No. 35103.)
Mannitol, glucose, lactose and raffinose in an Asiago Cheese Bagel were separated by gradient elution in 5 minutes on a Prevail Carbohydrate ES, 100 x 7mm Rocket column (Part No. 35103.)
Glucose, sucrose and lactose in a malted milk ball were separated by gradient elution in 7 minutes on a Prevail Carbohydrate ES, 100 x 7mm Rocket column (Part No. 35103.)
Sorbitol, fructose, maltose, maltotriose, maltotetraose and maltopentaose in raspberry jam were separated by gradient elution in 7 minutes on a Prevail Carbohydrate ES, 100 x 7mm Rocket column (Part No. 35103.)
Fructose, glucose and sucrose in clover honey were separated by gradient elution in around 10 minutes on a Prevail Carbohydrate ES, 5µm, 250 x 4.6mm column (Part No. 35101).
Key Analyte(s)
arabitol
fructose
glucose (dextrose)Glucose (dextrose)is a simple sugar used in foods and drinks. It is most often measured using HPLC and GC/MS.
Iso-erythritol
lactoseLactose (milk sugar) is a disaccharide found in milk and used pharmaceutical tablets. It is most often measured using HPLC and GC/MS.
maltitol
maltopentaose
maltose
maltotetraose
More AnalytesEquipment
Alltech Prevail Carbohydrate ES ColumnsAlltech Prevail Carbohydrate ES columns are a line of polymer based reversed phase HPLC columns in the Alltech Prevail family for use in determining carbohydrates including mono- and oligosaccharides and sugar alcohols. They are available with a single p
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
