Application Notes
Corn Syrup Oligomers by HPLC/ELSD
Oligosaccharide mixtures used as sweeteners were separated by reversed phase HPLC and detected by evaporative light scattering.
Carbohydrate Profiles-Confectionary Sweeteners, Alltech Application Note 0019E, January 18, 2002.
Oligosaccharide sweeteners in corn syrup and in commercial maltodextrin were separated isocratically and by gradient elution in around 25 minutes by reversed phase HPLC on a Prevail Carbohydrate ES, 5µm, 250 x 4.6mm column (Part No. 35101) using acetonitrile/water and detected by evaporative light scattering using an Alltech 2000 ELSD (Evaporative Light Scattering Detector).
Dextrose, maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose and maltooctaose were separated isocratically while these oliogomers and others in the series were separated by gradient elution. These other oligomers were maltononaose, maltodecaose, maltoundecaose, maltododecaose, maltotridecaose, maltotetradecaose and maltopentadecaose.
The maltodextrin was separated by gradient elution. Glucose (DPI), DP3 (1,6-alpha; 1,4 alpha), DP6 (1,6-alpha; 1,4 alpha), DP13 and DP18 were identified.
In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.
Key Analyte(s)
glucose (dextrose)Glucose (dextrose)is a simple sugar used in foods and drinks. It is most often measured using HPLC and GC/MS.
Glucose (DP1)
Glucose (DP13)
Glucose (DP18)
Glucose DP3 (1,6-alpha; 1,4 alpha)
Glucose DP6 (1,6-alpha; 1,4 alpha)
maltodecaose
maltodextrin
maltododecaose
More AnalytesEquipment
Alltech Prevail Carbohydrate ES ColumnsAlltech Prevail Carbohydrate ES columns are a line of polymer based reversed phase HPLC columns in the Alltech Prevail family for use in determining carbohydrates including mono- and oligosaccharides and sugar alcohols. They are available with a single p
Technique
HPLCHigh Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.
Source
Alltech Associates, Inc.Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.
