Application Notes
MMDA/MDA (Ecstasy) Determination by GC and HPLC
MDMA (3,4-methylenedioxymethamphetamine), an illegal drug also known as Ecstasy, and several of its analogs were separated by both GC/FID and HPLC/UV.
MDMA/MDA Application Note, Alltech Application Note #AN102, July 18, 2002.
MDMA (3,4-methylenedioxymethamphetamine), an illegal drug also known as Ecstasy, and several of its analogs were separated by both GC/FID and HPLC/UV.
The GC separation was accomplished on an Alltech Heliflex® ATTM-5ms, 15m x 0.25mm x 0.25µm capillary column (Part No. 15801) isothermally at 160°C using helium carrier gas. Detection was by FID at a temperature of 300°C. The separation of 3,4-MDMA, 2,3-MDMA, 2,3-MDA and 3,4-MDA took about 4 minutes.
The HPLC separation of these same four compounds in less than three minutes was accomplished on a Platinum® Silica, 3µm, 100 x 4.6mm reversed phase column (Part No. 32524) using a mobile phase of (75:25) acetonitrile/1% ammonium hydroxide in methanol and a flow rate of 2.0mL/min. Detection was by UV at 280nm.
Key Analyte(s)
2,3-MDA2,3-MDA (2,3-methylenedioxyamphetamine)an analog of the illegal drug Ecstasy. It is most often measured in blood or urine using HPLC and GC/FID.
2,3-MDMA2,3-MDMA (2,3-methylenedioxymethamphetamine)is an isomer of the illegal hallucinogenic drug Ecstasy (3,4-MDMA). It is most often measured in blood or urine using HPLC and GC/FID.
3,4-MDA3,4-MDA (3,4-methylenedioxyamphetamine) is an analog of the illegal drug Ecstasy. It is most often measured in blood or urine using HPLC and GC/FID.
3,4-MDMA (Ecstasy)3,4-MDMA (3,4-methylenedioxymethamphetamine, Ecstasy)is an illegal hallucinogenic drug. It is most often measured in blood or urine using HPLC and GC/FID.
Equipment
Alltech Heliflex® AT-5ms Capillary ColumnsAlltech Heliflex® AT-5ms capillary columns are a line of non-polar fused silica GC columns manufactured and sold by Alltech Associates. They are specifically designed for use with mass spectrometers.
Technique
GC/FID, HPLC/UV
Source
