Metal Chelates of 1-(2-pyridylazo)-2-naphthol (PAN) by HPLC

A mixture of four metal chelates of 1-(2-pyridylazo)-2-naphthol (PAN) was separated using a GROM-SIL 120 ODS-5 ST column and detected by visible spectroscopy at 565nm.

Metal Chelates of 1-(2-pyridylazo)-2-naphthol (PAN), Grom Application Number 01022

A mixture of four metal chelates of 1-(2-pyridylazo)-2-naphthol (PAN) was separated using a GROM-SIL 120 ODS-5 ST column (150 x 4mm, Part No. GSOD50512S1504) by isocratic elution with 10mM tetrabutylammonium bromide, 0.01% PAN and acetonitrile 30/70 v/v in around 25 minutes and detected by visible spectroscopy at 565nm. The compounds separated were Cu II-PAN, Ni II-PAN, Fe III-PAN and Zn II-PAN.