Polyamines in Decaying Fish by FMOC/EVE Derivatization

Five polyamines in a decaying fish fillet were separated using a GROM-SIL Polyamine-1 column after precolumn derivatization with FMOC/EVE and detected by fluorescence spectroscopy at 263 and 310nm.

Determination of Polyamines in Decaying Fish Fillet Applying Precolumn Derivatisation with FMOC/EVE and HPLC, Grom Application Number 10059

Five polyamines in a decaying fish fillet were separated using a GROM-SIL Polyamine-1 column (200 x 4mm, Part No. GSPO10510S2004) by gradient elution with 50 mM sodium acetate, pH 4.0 and acetonitrile 80/20 and 50 mM sodium acetate, pH 4.0 and acetonitrile = Mai 95 in around 15 minutes and detected by fluorescence spectroscopy at 263 and 310nm. The polyamines separated were putrescine, cataverine, histamine, spermidine and spermine.