Application Notes

Carbohydrates in Fruit Juices by HPLC/ELSD

By varying the mobile phase, six fruit sugars were separated by reversed phase HPLC and detected by evaporative light scattering.

Carbohydrates – Fruit Juices, Alltech Application Note 0012E, May 14, 2003.

Mixture of sugars found in fruit juices were separated isocratically in around 25 minutes by reversed phase HPLC on a Prevail Carbohydrate ES, 5µm, 250 x 4.6mm column (Part No. 35101) using acetonitrile/methanol/water. They were detected by evaporative light scattering using an Alltech 500 ELSD (Evaporative Light Scattering Detector).

By varying the mobile phase composition, fructose, glucose, sucrose, maltose and lactose were separated in one elution and fructose, glucose, sucrose and raffinose in another elution.

In ELSD, the mobile phase is first evaporated. Solid particles remaining from the sample are then carried in the form of a mist into a cell where they are detected by a laser.

Key Analyte(s)

fructose

glucose (dextrose)

Glucose (dextrose)is a simple sugar used in foods and drinks. It is most often measured using HPLC and GC/MS.

lactose

Lactose (milk sugar) is a disaccharide found in milk and used pharmaceutical tablets. It is most often measured using HPLC and GC/MS.

maltose

raffinose

sucrose

Equipment

Alltech Prevail Carbohydrate ES Columns

Alltech Prevail Carbohydrate ES columns are a line of polymer based reversed phase HPLC columns in the Alltech Prevail family for use in determining carbohydrates including mono- and oligosaccharides and sugar alcohols. They are available with a single p

Technique

HPLC

High Performance Liquid Chromatography (HPLC)is a technique for separating components in a sample on the basis of interactions between the component, a liquid mobile phase and a solid stationary phase.

Source

Alltech Associates, Inc.

Alltech Associates, Inc., one of the leading suppliers of HPLC columns, manufactures and distributes HPLC, GC and IC columns, accessories and consumables and maintains a large searchable database of application notes and chromatograms on its website.