Amino Acid Analysis After Precolumn Derivatization

Nineteen amino acids in a standard mixture were separated using a GROM-SIL OPA-1 column after precolumn derivatization with OPA /mercaptopropionic acid and detected by fluorescence spectroscopy at 330 and 450nm.

Amino Acid Analysis after Precolumn Derivatisation with OPA/Mercaptopropionic Acid – Extended Standard, Grom Application Number 10062

Nineteen amino acids in a standard mixture were separated using a GROM-SIL OPA-1 column (150 x 4mm, Part No. GSOP10308S1504) after precolumn derivatization with OPA/mercaptopropionic acid by gradient elution with 25mM sodium phosphate, pH 7.2 and THF 995/5 and 25mM sodium phosphate, pH 7.2, methanol and acetonitrile 50/35/15 in around 41 minutes and detected by fluorescence spectroscopy at 330 and 450nm. The amino acids separated were aspartic acid, glutamic acid, asparagine, serine, glutamine, glycine, threonine, histidine, citrulline, 3-methylhistidine, alanine, taurine, arginine, alpha-aminobutyric acid, tyrosine, valine, methionine, nor-valine and tryptophan.